3K1X
Acidic Fibroblast Growth Factor (FGF-1) complexed with dobesilate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 97.022, 47.349, 97.975 |
| Unit cell angles | 90.00, 106.64, 90.00 |
Refinement procedure
| Resolution | 24.480 - 1.980 |
| R-factor | 0.23041 |
| Rwork | 0.227 |
| R-free | 0.28958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1axm |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.273 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.070 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.050 | 0.342 |
| Number of reflections | 59016 | |
| <I/σ(I)> | 16 | 4.5 |
| Completeness [%] | 98.6 | 92.8 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | Crystals of thecomplex between FGF-1 and 2,5-DHPS (2,5-dihydroxyphenylsulfonate) were grown using the sitting drop vapour method at 295 K. Equal volumes of protein and inhibitor solutions, 0.75 and 1.5mM, respectively were mixed with drops containing 60% sodium/potassium tartrate buffered with 5mM sodium phosphate [pH 7.8]. The drops were equilibrated against 0.2ml of 1.3M Li2SO4 and typical crystals grew within two weeks with approximate dimensions of 0.7 x 0.5 x 0.2 mm. , VAPOR DIFFUSION, SITTING DROP |
