3K1E
Crystal structure of odorant binding protein 1 (AaegOBP1) from Aedes aegypti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.248, 47.870, 69.082 |
| Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
| Resolution | 68.680 - 1.850 |
| R-factor | 0.154 |
| Rwork | 0.151 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.188 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 19077 | |
| <I/σ(I)> | 20.35 | 4.4 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 7.3 | 7.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 2 microliter drops, with equal volumes of protein at 50 mg/mL and reservoir solution containing 28% PEG 8000, 250 mM MgCl2, 50 mM Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
