3JZQ

Human MDMX liganded with a 12mer peptide inhibitor (pDIQ)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	44.925, 53.735, 87.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.500 - 1.800
Rwork	0.208
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dab
RMSD bond length	0.016
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.062	0.470
Number of reflections	20193
<I/σ(I)>	27.5	3.7
Completeness [%]	99.7	99
Redundancy	6.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	292	2.1 M ammonium sulfate, 10 mM Tris HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K