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3JUT

Acidic Fibroblast Growth Factor (FGF-1) complexed with gentisic acid

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE BM16
Synchrotron siteESRF
BeamlineBM16
Temperature [K]100
Detector technologyCCD
Collection date2008-12-07
DetectorADSC QUANTUM 210r
Wavelength(s)0.979
Spacegroup nameP 1 2 1
Unit cell lengths97.658, 47.685, 98.413
Unit cell angles90.00, 106.45, 90.00
Refinement procedure
Resolution37.010 - 2.250
R-factor0.21887
Rwork0.216
R-free0.27406
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1axm
RMSD bond length0.026
RMSD bond angle2.106
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareAMoRE
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]47.7002.330
High resolution limit [Å]2.2102.210
Rmerge0.0820.328
Number of reflections41838
<I/σ(I)>5.61.6
Completeness [%]98.894.6
Redundancy3.33.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.8295Equal volumes of protein and inhibitor solutions, 0.75 and 1.5 mM,respectively were mixed with drops containing 60% sodium/potassium tartrate buffered with 5 mM sodium phosphate [pH 7.8]. The drops were equilibrated against 200 ml of 1.3M Li2SO4 and typical crystals grew within two weeks , VAPOR DIFFUSION, SITTING DROP, temperature 295K

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