3JUG
Crystal structure of endo-beta-1,4-mannanase from the alkaliphilic Bacillus sp. N16-5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.033, 63.312, 83.342 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.176 - 1.600 |
R-factor | 0.1758 |
Rwork | 0.174 |
R-free | 0.21470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wky |
RMSD bond length | 0.007 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.290 | 1.710 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 38945 | |
Completeness [%] | 92.7 | 65.44 |
Redundancy | 4.68 | 1.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.5M Ammonium Sulphate, 0.1M sodium citrate, 1.2M Lithium Sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |