1WKY

Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module

Summary for 1WKY

• Entry DOI: 10.2210/pdb1wky/pdb
• Descriptor: endo-beta-1,4-Mannanase, CHLORIDE ION, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: tim barrel, catalytic domain, cbm, hydrolase
• Biological source: Bacillus sp.
• Total number of polymer chains: 1
• Total formula weight: 51189.61

Authors

Akita, M.,Takeda, N.,Hirasawa, K.,Sakai, H.,Kawamoto, M.,Yamamoto, M.,Grant, W.D.,Hatada, Y.,Ito, S.,Horikoshi, K. (deposition date: 2004-06-15, release date: 2005-06-15, Last modification date: 2023-10-25)

Primary citation

Akita, M.,Takeda, N.,Hirasawa, K.,Sakai, H.,Kawamoto, M.,Yamamoto, M.,Grant, W.D.,Hatada, Y.,Ito, S.,Horikoshi, K.
Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate.
Acta Crystallogr.,Sect.D, 60:1490-1492, 2004

PubMed Abstract: An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain.

PubMed: 15272186
DOI: 10.1107/S0907444904014313

Experimental method

X-RAY DIFFRACTION (1.65 Å)