3JTL
Crystal structure of archaeal 20S proteasome in complex with mutated P26 activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 255.826, 126.582, 182.170 |
Unit cell angles | 90.00, 92.95, 90.00 |
Refinement procedure
Resolution | 29.850 - 3.200 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yar |
RMSD bond length | 0.015 |
RMSD bond angle | 1.467 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.257 | 0.642 |
Number of reflections | 95561 | |
<I/σ(I)> | 4.2 | |
Completeness [%] | 98.9 | 90.9 |
Redundancy | 6.8 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 294 | pH 5.6, PEG 1000, Sodium phosphate citrate, liso4, imidazole, vapor diffusion, hanging drop, temperature 294K, VAPOR DIFFUSION, HANGING DROP |