3JTD
Calcium-free Scallop Myosin Regulatory Domain with ELC-D19A Point Mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-12-25 |
Detector | PRINCETON 2K |
Wavelength(s) | 0.908 |
Spacegroup name | P 1 |
Unit cell lengths | 42.000, 52.780, 58.510 |
Unit cell angles | 113.36, 91.56, 100.60 |
Refinement procedure
Resolution | 34.890 - 2.570 |
R-factor | 0.2329 |
Rwork | 0.233 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wdc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.000 |
High resolution limit [Å] | 2.570 |
Number of reflections | 13187 |
<I/σ(I)> | 12.298 |
Completeness [%] | 90.5 |
Redundancy | 2.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 277 | 100 mM bicine pH 8.3, 45 mM ammonium sulfate, 5 mM magnesium chloride, 2.5 mM EGTA, 3.0 mM sodium azide, 15% (wt/vol) PEG 4000, 4% (vol/vol) PEG 200, 6% (wt/vol) 2,3-butanediol, Hanging drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP |