3JSD
Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-03-25 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 80.843, 80.843, 38.842 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.370 - 2.500 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1trz |
RMSD bond angle | 0.009 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.370 | 2.660 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.071 | 0.254 |
Number of reflections | 3190 | |
Completeness [%] | 97.6 | 95.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 298 | 0.02M Tris-HCl, 0.05M Sodium citrate, 5% Acetone, 0.03% Phenol, 0.01% Zinc acetate, pH 8.0, VAPOR DIFFUSION, temperature 298K |