3JSB
Crystal structure of the N-terminal domain of the Lymphocytic Choriomeningitis Virus L protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2008-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9835 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 145.010, 159.350, 52.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.590 - 2.130 |
| R-factor | 0.1997 |
| Rwork | 0.199 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.250 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.039 | |
| Total number of observations | 215459 | |
| Number of reflections | 33999 | |
| <I/σ(I)> | 27.1 | 1.9 |
| Completeness [%] | 97.9 | 97.8 |
| Redundancy | 6.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.2M-0.8M LiSo4, 50mM Citrate, 5-10% Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
