3JPX

EED: A Novel Histone Trimethyllysine Binder Within The EED-EZH2 Polycomb Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-04-06
Detector	RIGAKU RAXIS
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	57.877, 85.298, 91.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.890 - 2.050
R-factor	0.16683
Rwork	0.164
R-free	0.21052
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qxv
RMSD bond length	0.023
RMSD bond angle	1.893
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.890
High resolution limit [Å]	2.050
Rmerge	0.154
Number of reflections	28626
<I/σ(I)>	5.5
Completeness [%]	98.3
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	3.5M NaF, 10 mM TCEP Chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K