EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | flash freezing |
EM Data Collection
| Microscope | JEOL 3200FS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN ULTRASCAN 1000 (2k x 2k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7 |
| Software | MDFF,RELION,SPIDER,PHENIX |
| Symmetry | C1 |
