3JCK
Structure of the yeast 26S proteasome lid sub-complex
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | 4 uL sample was applied to the grid, blotted for 2 seconds, and plunged into liquid ethane. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 3432 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Software | CTFFIND,FindEM,Appion,RELION,PHENIX |
| Symmetry | C1 |
