3JCK
Structure of the yeast 26S proteasome lid sub-complex
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
Method | 4 uL sample was applied to the grid, blotted for 2 seconds, and plunged into liquid ethane. |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 (4k x 4k) |
Number of Images | 3432 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Software | CTFFIND,FindEM,Appion,RELION |
Symmetry | C1 |