3JCH
Cryo-EM structure of the magnesium channel CorA in the magnesium-free, asymmetric open state II
EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
Method | Grids were blotted at 4 degrees Celsius for 7 seconds after a 10-second pre-blotting period, then plunge-frozen in liquid ethane. |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF |
Detector | GATAN K2 QUANTUM (4k x 4k) |
Number of Images | 2498 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 7.06 |
Software | Coot,PHENIX,UCSF Chimera,RELION |
Symmetry | C1 |