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3JCF

Cryo-EM structure of the magnesium channel CorA in the closed symmetric magnesium-bound state

EM specimen
VitrificationYes
InstrumentLEICA EM GP
MethodGrids were blotted at 4 degrees Celsius for 7 seconds after a 10-second pre-blotting period, then plunge-frozen in liquid ethane.
EM Data Collection
MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
Energy FilterGIF
DetectorGATAN K2 QUANTUM (4k x 4k)
Number of Images959
3D Reconstruction
MethodSINGLE PARTICLE
Resolution (Å)3.8
SoftwareCoot,PHENIX,UCSF Chimera,RELION
SymmetryC5

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PDB entries from 2024-11-13

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