3JCF
Cryo-EM structure of the magnesium channel CorA in the closed symmetric magnesium-bound state
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
| Method | Grids were blotted at 4 degrees Celsius for 7 seconds after a 10-second pre-blotting period, then plunge-frozen in liquid ethane. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
| Number of Images | 959 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.8 |
| Software | Coot,PHENIX,UCSF Chimera,RELION |
| Symmetry | C5 |
