3JCA
Core model of the Mouse Mammary Tumor Virus intasome
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | 3 uL sample was applied to grid, adsorbed for 30 seconds, blotted, and plunge-frozen in liquid ethane. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 2714 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.8 |
| Software | Rosetta,FREALIGN |
| Symmetry | C2 |
