3JC2
The structure of the mammalian Sec61 channel opened by a signal sequence
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | 3 uL sample was added to the grid, incubated for 30 seconds at 4 C, blotted for 9 seconds |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Software | Coot,REFMAC,UCSF Chimera,RELION |
| Symmetry | C1 |
