3JC2
The structure of the mammalian Sec61 channel opened by a signal sequence
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | 3 uL sample was added to the grid, incubated for 30 seconds at 4 C, blotted for 9 seconds |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.6 |
Software | Coot,REFMAC,UCSF Chimera,RELION |
Symmetry | C1 |