3JB7
In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 4907 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Software | FREALIGN |
| Symmetry | C1 |
