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3JAT

Cryo-EM structure of GMPCPP-microtubule (14 protofilaments) decorated with kinesin

EM specimen
VitrificationYes
InstrumentFEI VITROBOT MARK II
MethodBlot once for 4 seconds before plunging.
EM Data Collection
MicroscopeFEI TITAN
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
DetectorGATAN K2 (4k x 4k)
Number of Images695
3D Reconstruction
MethodHELICAL
Resolution (Å)3.5
SoftwareEMAN,FREALIGN

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PDB entries from 2024-03-27

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