3JAM
CryoEM structure of 40S-eIF1A-eIF1 complex from yeast
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | Blot for 2.5 to 3 seconds before plunging |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | FEI FALCON II (4k x 4k) |
Number of Images | 2056 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.46 |
Software | Coot,REFMAC,UCSF Chimera,RELION |
Symmetry | C1 |