3JAM
CryoEM structure of 40S-eIF1A-eIF1 complex from yeast
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 2.5 to 3 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
| Number of Images | 2056 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.46 |
| Software | Coot,REFMAC,UCSF Chimera,RELION |
| Symmetry | C1 |
