3J9T
Yeast V-ATPase state 1
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Blot for 23 seconds before freezing |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 3685 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.9 |
| Software | MDFF,UCSF Chimera,TMaCS,RELION |
| Symmetry | C1 |
