3J9S
Single particle cryo-EM structure of rotavirus VP6 at 2.6 Angstrom resolution
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
Method | Blot for 4-6 seconds |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.6 |
Software | Coot,FREALIGN,IMAGIC,TIGRIS |
Symmetry | I |