3J9P
Structure of the TRPA1 ion channel determined by electron cryo-microscopy
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | Blot for 7 seconds before plunging. |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | GATAN K2 (4k x 4k) |
Number of Images | 1160 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.24 |
Software | RELION |
Symmetry | C4 |