3J8X
High-resolution structure of no-nucleotide kinesin on microtubules
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | No glow discharge was applied. After sample application to grid, liquid was mostly 'wicked' away by edgewise application of filter paper. Subsequently, blotting and plunge freezing were performed with ~0.5 second delay after blotting but prior to plunging. |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 5 |
| Software | MDFF,FREALIGN,SPIDER |
