3J8H

Structure of the rabbit ryanodine receptor RyR1 in complex with FKBP12 at 3.8 Angstrom resolution

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK III
Method	protein solution was applied to glow-discharged carbon coated grid and blotted for 2 seconds before plunging

EM Data Collection

Microscope	FEI POLARA 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2
Detector	FEI FALCON II (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.8
Software	CTFFIND,Coot,PHENIX,REFMAC,RELION
Symmetry	C4