3J8F
Cryo-EM reconstruction of poliovirus-receptor complex
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Sample mixed and frozen within 2 minutes. |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.26 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 285 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Software | Coot,REFMAC,SPDBV,FREALIGN,RELION |
| Symmetry | I |
