EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 120 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 9.3 |
Software | UCSF Chimera,RELION,SPIDER |
Symmetry | C1 |