3J6P
Pseudo-atomic model of dynein microtubule binding domain-tubulin complex based on a cryoEM map
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 3.5 seconds before plunging |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 1.6 |
| Detector | TVIPS TEMCAM-F415 (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 8.2 |
| Software | DireX,Flex-EM,SPIDER |
