3J6F
Minimized average structure of GDP-bound dynamic microtubules
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
| Method | The grid was blotted for 2 seconds before plunging. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 219 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.9 |
| Software | Rosetta,EMAN,FREALIGN,SPARX |
