3J5S
EttA binds to ribosome exit site and regulates translation by restricting ribosome and tRNA dynamics
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Wait time 30 sec, blot time 8 sec, at 4 degrees Celsius |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 1816 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.5 |
| Software | NAMD,RELION,SPIDER |
| Symmetry | C1 |
