3J46
Structure of the SecY protein translocation channel in action
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 160 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 4900 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 10.1 |
| Software | MDFF,UCSF Chimera,EMAN |
| Symmetry | C1 |
