3J2W
Electron cryo-microscopy of Chikungunya virus
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 2 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GENERIC FILM |
| Number of Images | 1532 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5 |
| Software | EMfit,EMAN,FREALIGN |
| Symmetry | I |
