3J0P
Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 10.6A cryo-em map: rotated PRE state 1
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | KODAK SO-163 FILM |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 10.6 |
Software | UCSF Chimera,SPIDER |
Symmetry | C1 |