3J0P

Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 10.6A cryo-em map: rotated PRE state 1

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	FEI POLARA 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2
Detector	KODAK SO-163 FILM

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	10.6
Software	UCSF Chimera,SPIDER
Symmetry	C1