3J0K
Orientation of RNA polymerase II within the human VP16-Mediator-pol II-TFIIF assembly
EM specimen
Vitrification | Yes |
Method | blot for 2 seconds, dry for 3 seconds before plunging |
Staining material | ammonium molybdate |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2 |
Detector | KODAK SO-163 FILM |
Number of Images | 106 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 36 |
Software | Situs,SPIDER |
Symmetry | C1 |