3J0K
Orientation of RNA polymerase II within the human VP16-Mediator-pol II-TFIIF assembly
EM specimen
| Vitrification | Yes |
| Method | blot for 2 seconds, dry for 3 seconds before plunging |
| Staining material | ammonium molybdate |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 106 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 36 |
| Software | Situs,SPIDER |
| Symmetry | C1 |
