3J0A
Homology model of human Toll-like receptor 5 fitted into an electron microscopy single particle reconstruction
EM specimen
| Vitrification | Yes |
| Staining | Yes |
| Staining material | Uranyl Formate |
EM Data Collection
| Microscope | FEI TECNAI 12 |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2.2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 26 |
| Software | MODELLER,REFMAC,IMAGIC,SPIDER |
| Symmetry | C1 |
