3J09
High resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopA
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | blot for 5 seconds before plunging |
EM Data Collection
| Microscope | FEI/PHILIPS CM200FEG |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 10 |
| Software | EMIP |
