3IVD
Putative 5'-Nucleotidase (c4898) from Escherichia Coli in complex with Uridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.597, 67.281, 86.417 |
| Unit cell angles | 74.04, 77.55, 83.76 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.21000 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.338 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.107 | 0.062 | 0.462 |
| Number of reflections | 76172 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 96.3 | 99 | 84.4 |
| Redundancy | 3.6 | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 30% PEG 4K, 0.1M 0.2M MgCl2, Tris-HCl pH 8.5, Vapor diffusion, Sitting drop, temperature 298K |
