3IRW
Structure of a c-di-GMP riboswitch from V. cholerae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1050, 1.1054, 1.0762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.461, 45.123, 76.573 |
Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
Resolution | 43.690 - 2.700 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.25100 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.953 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.072 | 0.382 |
Number of reflections | 8935 | |
<I/σ(I)> | 12.5 | 1.9 |
Completeness [%] | 94.8 | 82.4 |
Redundancy | 2.7 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 298 | 22% PEG550mme, 50 mM MES, pH 6.0, 5 mM MgSO4, 300 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |