Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 92.810, 92.810, 60.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.200 - 2.230 |
| R-factor | 0.14226 |
| Rwork | 0.135 |
| R-free | 0.20928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 2.350 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.046 | 0.104 |
| Number of reflections | 12667 | |
| <I/σ(I)> | 26.5 | 12.5 |
| Completeness [%] | 94.7 | 67.6 |
| Redundancy | 6.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.66 | 281 | Crystal were obtained from a 400 nL drop formed from 200 nL of protein solution (11.2 mg/mL, in 10 mM Tris-HCl, pH 8.0) and 200 nL of precipitant (polyethylene glycol 3350 15.7% w/v, malic acid 100 mM, pH 5.66). The drop was incubated at 281K. The reservoir (80 micro-L) contained polyethylene glycol 3350 (15.7% w/v) and malic acid (100 mM, pH 5.66)., VAPOR DIFFUSION, SITTING DROP |
