3IR2
Crystal structure of the APOBEC3G catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90020 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.338, 72.532, 97.433 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.16783 |
| Rwork | 0.166 |
| R-free | 0.20823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Highly truncated model based on PDB Entry 3E1U |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.220 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.075 | 0.365 |
| Number of reflections | 23001 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 97.1 | 97.4 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES, 10% PEG 4000, 0.1M magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
