3IQZ
Structure of F420 dependent methylene-tetrahydromethanopterin dehydrogenase in complex with methylene-tetrahydromethanopterin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.008 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.810, 167.700, 95.860 |
Unit cell angles | 90.00, 101.25, 90.00 |
Refinement procedure
Resolution | 94.000 - 2.100 |
R-factor | 0.19981 |
Rwork | 0.198 |
R-free | 0.22647 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qv9 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.570 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 109528 | |
<I/σ(I)> | 16.1 | 2.9 |
Completeness [%] | 96.7 | 96.4 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG400, 0.1M MES, 0.1M sodium acetate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |