3IQV
Crystal Structure of human 14-3-3 sigma in Complex with Raf1 peptide (6mer) and stabilisator Fusicoccin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-16 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.370, 111.750, 62.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.650 - 1.200 |
R-factor | 0.12481 |
Rwork | 0.123 |
R-free | 0.15322 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ywt |
RMSD bond length | 0.026 |
RMSD bond angle | 2.184 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.250 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 89939 | |
<I/σ(I)> | 19.1 | 4.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.3 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20mM Hepes/NaOH pH 7.5, 2mM MgCl2 and 2 mM DTT, set up for crystallization in 0.1 M Hepes/NaOH ph 7.5, 0.2 M CaCl2, 28% PEG 400, 5% glycerol, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |