3IPM
Crystal Structure of Archaeal 20S Proteasome in Complex with the C-terminus of PAN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 166.890, 166.890, 412.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.510 - 4.000 |
| R-factor | 0.25 |
| Rwork | 0.249 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ya7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.500 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.226 | 0.449 |
| Number of reflections | 48850 | |
| <I/σ(I)> | 2.9 | 2.7 |
| Completeness [%] | 98.4 | 98.6 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.1 M MES pH6.8, 20% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
