3IPD

Helical extension of the neuronal SNARE complex into the membrane, spacegroup I 21 21 21

Replaces:  3HD9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9789
Spacegroup name	I 21 21 21
Unit cell lengths	109.852, 215.680, 262.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.884 - 4.800
R-factor	0.3051
Rwork	0.304
R-free	0.33180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hd7
RMSD bond length	0.004
RMSD bond angle	0.696
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.4_4))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.880
High resolution limit [Å]	4.800	4.800
Number of reflections	15646
<I/σ(I)>	11.1	2.1
Completeness [%]	99.9	99.9
Redundancy	7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	16% MPD, 0.1M TRIS, 0.05M MAGNESIUM CHLORIDE, 3% SORBITOL, pH 8.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K