3IOT
Huntingtin amino-terminal region with 17 Gln residues - crystal C92-b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.250, 101.360, 137.030 |
| Unit cell angles | 90.00, 91.83, 90.00 |
Refinement procedure
| Resolution | 37.360 - 3.500 |
| R-factor | 0.24957 |
| Rwork | 0.247 |
| R-free | 0.29516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ior |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.710 |
| Number of reflections | 40594 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 12% PEG 4000, 200 mM Zn acetate, 200 mM Sodium acetate, 100 mM Sodium Cacodylate pH 6.5-7.4, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
