3ILU
Crystal structure of the AMPA subunit GluR2 bound to the allosteric modulator, hydroflumethiazide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 47.289, 114.194, 163.732 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.636 - 2.003 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dp6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.190 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.145 | 0.781 |
Number of reflections | 60617 | |
<I/σ(I)> | 6.1 | 2.317 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.1 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16-18 PEG8K, 0.1 M Na Cacodylate, 0.1-0.15 zinc acetate, pH 6.5, vapor diffusion, hanging drop, temperature 277K |