3IID
Crystal structure of the macro domain of human histone macroH2A1.1 in complex with ADP-ribose (form A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.973 |
Spacegroup name | P 61 |
Unit cell lengths | 86.260, 86.260, 51.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.860 - 1.900 |
R-factor | 0.17769 |
Rwork | 0.176 |
R-free | 0.21467 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zr3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.680 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 17128 | |
<I/σ(I)> | 23.7 | 3.6 |
Completeness [%] | 99.5 | 94.4 |
Redundancy | 10 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 35% PEG 1000, 0.2 M K2NO3, VAPOR DIFFUSION, SITTING DROP, temperature 294K |