3II0
Crystal structure of human Glutamate oxaloacetate transaminase 1 (GOT1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.531, 107.347, 239.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.040 - 2.050 |
| R-factor | 0.18259 |
| Rwork | 0.180 |
| R-free | 0.22316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ajs |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.040 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.108 | 0.780 |
| Number of reflections | 127732 | |
| <I/σ(I)> | 8.7 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.25M Na_K_tartrate; 15w/v PEG_3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
