3IH2
TM1030 crystallized at 323K
Experimental procedure
| Experimental method | MOLECULAR REPLACEMENT |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 55.929, 66.935, 55.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.720 - 2.300 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z77 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.720 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.067 | 0.514 |
| Number of reflections | 9996 | |
| <I/σ(I)> | 39.901 | 3.6 |
| Completeness [%] | 98.3 | 97.4 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 323 | 30% w/v PEG 2000 MME, 0.1M KSCN, , VAPOR DIFFUSION, HANGING DROP, temperature 323K |
