3ICE
Rho transcription termination factor bound to RNA and ADP-BeF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.230, 127.030, 127.170 |
| Unit cell angles | 60.48, 90.26, 89.77 |
Refinement procedure
| Resolution | 41.880 - 2.800 |
| R-factor | 0.271 |
| Rwork | 0.270 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PVO AND UNPUBLISHED STRUCTURE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.970 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.079 | 0.342 |
| Number of reflections | 87864 | |
| <I/σ(I)> | 15.5 | 3.8 |
| Completeness [%] | 94.6 | 66.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 291 | 2.5% MPD, 50mM HEPES, 5mM Tris-HCL, 160mM sodium chloride, 1.25mM magnesium chloride, 5mM spermidine-HCL, 0.5mM TCEP, 1.25mM ADP-BeF3,, pH 7.9, MICROBATCH, temperature 291K |
